Colchicine-Mediated Polyploidization for Improvement of French Marigold Traits
Keywords:
Colchicine, Polyploidization, Tagetes Patula, French Marigold, Morphological TraitsAbstract
This study evaluated the effects of colchicine concentration and soaking duration on vegetative morphology of French marigold (Tagetes patula L.) cv. Janie Spry, a naturally allotetraploid species (2n = 4x = 48). A 4×3 factorial completely randomized design was employed with four colchicine concentrations (0%, 0.1%, 0.2%, 0.3%) and three soaking durations (4, 8, 12 hours), totaling 12 treatments with three replications (n = 36). Germinated seeds at the radicle stage were soaked in colchicine solutions and grown in a greenhouse. Survival rate, plant height, stem diameter, and number of shoots were recorded during the vegetative phase. Two-way ANOVA revealed highly significant interaction effects (p<0.001) of concentration and duration on survival rate and shoot number, while plant height and stem diameter were not significantly affected. Survival declined sharply at higher concentrations with prolonged soaking, reaching only 30% at 0.3% colchicine for 12 hours. The 0.2% colchicine at 4-hour soaking treatment was identified as optimal, maintaining 90% survival while increasing shoot number by 37.7% relative to the control (30.7 vs. 22.3 shoots plant⁻¹). The highest shoot count (34.3 shoots plant⁻¹) occurred at 0.3% for 12 hours but was accompanied by unacceptable seedling mortality. These results indicate that moderate colchicine concentrations with short exposure offer an effective balance between morphological modification and seedling viability in T. patula. Cytological confirmation of ploidy levels through chromosome counting and flow cytometry is ongoing.
References
[1] D. K. Mishra, S. Singh, and P. Singh, “Therapeutic benefits and processing of marigold (Tagetes species): A review,” Indian J. Health Care Med. Pharm. Pract., vol. 5, no. 1, pp. 148–166, 2024. doi: 10.59551/IJHMP/25832069/2024.5.1.190
[2] G. S. Abrol, P. Sharma, A. Kumar, and G. Sharma, “Different Preharvest Factors and Their Role in the Floriculture,” in Ornamental Horticulture: Latest Cultivation Practices and Breeding Technologies, B. Bhargava, P. Kumar, and V. Verma, Eds., Singapore: Springer Nature Singapore, 2024, pp. 227–242. doi: 10.1007/978-981-97-4028-4_12. doi: 10.1007/978-981-97-4028-4.
[3] A. Uniyal and A. Kumar, “Minor flowers of European and American countries,” in Edible flowers, Elsevier, 2024, pp. 385–430. Accessed: Mar. 18, 2026. 10.1016/B978-0-443-13769-3.00020-0
[4] F. Kurniati, “Potensi Bunga Marigold (Tagetes erecta L.) Sebagai Salah Satu Komponen Pendukung Pengembangan Pertanian,” Media Pertan., vol. 6, no. 1, May 2021, doi: 10.37058/mp.v6i1.3010.
[5] A. Buchori et al., “assessment of tagetes patula mutants and its wild type for flower morphology, polyphenol contents, and antioxidant activity,” sabrao j. Breed. Genet., vol. 56, pp. 1147–1158, Jun. 2024. 10.54910/sabrao2024.56.3.21
[6] A. Manzoor, T. Ahmad, M. A. Bashir, I. A. Hafiz, and C. Silvestri, “Studies on Colchicine Induced Chromosome Doubling for Enhancement of Quality Traits in Ornamental Plants,” Plants, vol. 8, no. 7, Jun. 2019, doi: 10.3390/plants8070194.
[7] Y. M. Ye, J. Tong, X. P. Shi, W. Yuan, and G. R. Li, “Morphological and cytological studies of diploid and colchicine-induced tetraploid lines of crape myrtle (Lagerstroemia indica L.),” Sci. Hortic., vol. 124, no. 1, pp. 95–101, 2010. 10.1016/j.scienta.2009.12.016
[8] F. Fakhrzad, A. Jowkar, A. Shekafandeh, and M. J. Kermani, “Tetraploidy induction enhances morphological, physiological and biochemical characteristics of wallflower (Erysimum cheiri (L.) Crantz),” Sci. Hortic., vol. 308, p. 111596, 2023. 10.1016/j.scienta.2022.111596
[9] K. Bhattarai, A. Kareem, and Z. Deng, “In vivo induction and characterization of polyploids in gerbera daisy,” Sci. Hortic., vol. 282, p. 110054, 2021. 10.1016/j.scienta.2021.110054
[10] M. C. Sattler, C. R. Carvalho, and W. R. Clarindo, “The polyploidy and its key role in plant breeding,” Planta, vol. 243, no. 2, pp. 281–296, Feb. 2016, doi: 10.1007/s00425-015-2450-x.
[11] W.-H. Eng and W.-S. Ho, “Polyploidization using colchicine in horticultural plants: A review,” Sci. Hortic., vol. 246, pp. 604–617, 2019. 10.1016/j.scienta.2018.11.010
[12] N. Ermawati, N. Sjamsijah, R. A. Syaban, H. Prasetyo, and M. Safi’i, “Induksi Poliploidi Menggunakan Perlakuan Kolkisin pada Anggrek Bulan (Phalaenopsis sp.) secara In Vivo,” Agriprima J. Appl. Agric. Sci., vol. 8, no. 1, pp. 61–70, 2024. 10.25047/agriprima.v8i1.657
[13] Y. Sajjad, M. J. Jaskani, A. Mehmood, I. Ahmad, and H. Abbas, “Effect of colchicine on in vitro polyploidy induction in African marigold (Tagetes erecta),” Pak J Bot, vol. 45, no. 3, pp. 1255–1258, 2013.
[14] R. S. Raghunath Sadhukhan, A. G. Arghadeep Ganguly, P. K. Singh, and H. K. Sarkar, “Study of induced polyploidy in African marigold (Tagetes erecta L.).,” 2014, Accessed: Mar. 18, 2026. 10.5555/20143382196
[15] F. U. Yongyao, Y. Liping, Z. Kaimin, and X. U. Wenji, “Induction and Characterization of Medicinal Polyploid Tagetes erecta,” Chin. J. Trop. Crops, vol. 42, no. 5, pp. 1318–1325, May 2021, doi: 10.3969/j.issn.1000-2561.2021.05.017.
[16] J. W. Towner, “cytogenetic studies on the origin of tagetes patula. I. Meiosis and morphology of diploid and allotetraploid t. Erecta × t. Tenuifolia,” Am. J. Bot., vol. 48, no. 9, pp. 743–751, Oct. 1961, doi: 10.1002/j.1537-2197.1961.tb11706.x.
[17] R. Cicevan et al., “Biological Traits and Genetic Relationships Amongst Cultivars of Three Species of Tagetes (Asteraceae),” Plants, vol. 11, no. 6, p. 760, Jan. 2022, doi: 10.3390/plants11060760.
[18] Y. N. SARI, S. I. AISYAH, D. SUKMA, and M. SYUKUR, “Increasing ploidy level of marigold (Tagetes patula Sudamala Barak) using colchicine with acute and chronic methods.,” Biodiversitas J. Biol. Divers., vol. 26, no. 6, 2025, Accessed: Mar. 18, 2026. 10.13057/biodiv/d260617
[19] J. Avdić, N. Sarajlić, B. Bečić, S. Murtić, and A. Turalija, “The effect of concentration, exposure time and type of colchicine treatment on the morphological characteristics of French marigold (Tagetes patula L. var. nana).,” 2013, Accessed: Mar. 18, 2026. 10.5555/20153005572
[20] A. Manzoor, T. Ahmad, M. A. Bashir, I. A. Hafiz, and C. Silvestri, “Studies on colchicine induced chromosome doubling for enhancement of quality traits in ornamental plants,” Plants, vol. 8, no. 7, p. 194, 2019. 10.3390/plants8070194
[21] A. Manzoor et al., “Assessment of biological damage and toxic potency of colchicine in gladiolus (Gladiolus grandiflorus) plants.,” 2023, Accessed: Mar. 18, 2026. 10.5555/20231604874
[22] Y. Sajjad, M. J. Jaskani, A. Mehmood, I. Ahmad, and H. Abbas, “Effect of colchicine on in vitro polyploidy induction in African marigold (Tagetes erecta),” Pak J Bot, vol. 45, no. 3, pp. 1255–1258, 2013.
[23] F. P. KARAGÖZ, A. Dursun, B. Dumlu, M. Karaşal, and H. Karagöz, “Colchicine-induced polyploidy and morphological changes in wild Silene compacta Fischer: Potential as an ornamental plant in Türkiye,” J. Biometry Stud., vol. 4, no. 2, pp. 79–90, 2024. 10.61326/jofbs.v4i2.04
[24] Y. M. Ye, J. Tong, X. P. Shi, W. Yuan, and G. R. Li, “Morphological and cytological studies of diploid and colchicine-induced tetraploid lines of crape myrtle (Lagerstroemia indica L.),” Sci. Hortic., vol. 124, no. 1, pp. 95–101, 2010.
[25] S. Nasirvand, R. A. Zakaria, N. Zare, and B. Esmaeilpoor, “Polyploidy induction in parsley (Petroselinum crispum L.) by colchicine treatment,” Cytologia (Tokyo), vol. 83, no. 4, pp. 393–396, 2018. 10.1508/cytologia.83.393
[26] W. H. Eng, W. S. Ho, and K. H. Ling, “In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment,” PeerJ, vol. 9, p. e12399, 2021. 10.7717/peerj.12399
[27] R. Sadhukhan, A. Ganguly, P. K. Singh, and H. K. Sarkar, “Study of Induced polyploidy in African marigold (Tagetes ecrecta L.),” Env. Ecol, vol. 32, no. 4, pp. 1219–1222, 2014. 10.5555/20143382196
[28] G. Bian et al., “Colchicine-induced polyploidization influences the morphological, physiological, and biochemical characteristics of Cyclocarya paliurus,” Plants, vol. 14, no. 17, p. 2778, 2025. 10.3390/plants14172778
[29] K. Bhattarai, A. Kareem, and Z. Deng, “In vivo induction and characterization of polyploids in gerbera daisy,” Sci. Hortic., vol. 282, p. 110054, 2021. 10.1016/j.scienta.2021.110054
[30] K. S. Koh, S. Gantait, M. F. Ismail, N. S. Naharudin, and U. R. Sinniah, “Induction of in vitro Tetraploids in Kaempferia parviflora Wall. ex Baker and its Characterization,” Vitro Cell. Dev. Biol. - Plant, vol. 61, no. 2, pp. 268–281, Apr. 2025, doi: 10.1007/s11627-024-10483-4.
[31] N. I. Jeloudar, E. Chamani, A. Shokouhian, and R. A. Zakaria, “Induction and identification of polyploidy by colchicine treatment in Lilium regale,” Cytologia (Tokyo), vol. 84, no. 3, pp. 271–276, 2019. 10.59551/IJHMP/25832069/2024.5.1.190
